Protein Purification Reagents and Kits
Bioss Vaccina virus
Vaccina virus Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications ELISA IHC Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
STEMCELL Technologies EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit is designed to isolate highly purified CD25+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. The kit targets CD25 regulatory T cells for positive selection with a PE-labeled antibody directed against the CD25 surface marker. Desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube.
STEMCELL Technologies EasySep™ Direct Human Total Lymphocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Total Lymphocyte Isolation Kit isolates highly purified lymphocytes directly from human whole blood by immunomagnetic negative selection. Isolated cells are untouched and available for direct use in flow cytometry, culture, DNA/RNA extraction or other downstream applications.
Creative Biomart m7GTP Agarose 1mL
Size :1 mL, Matrix :Highly cross-linked agaroseAverage particle size :45 - 165 μmLigand :m7GTPLigand concentration :5 mol m7GTP/ml gelRecommended flow rate :11.5 cm/hChemical stability :Stable to all solutions commonly used in gel filtration including 8 M urea and 6 M guanidine hydrochloride.Not stable in organic solvents!pH working range :7.5pH CIP range :4 - 9Shelf life :12 months
Biotium Primary Antibody CD8a SK1 CF503R 1/EA
CD8a (clone SK1) is a mouse monoclonal antibody that recognizes the human protein CD8 CD8a is a common marker of cytotoxic (Killer) T cells and is a T cell coreceptor This CF503R antibody conjugate has been validated in flow cytometry
ACROBiosystems Human GLP1R Protein, His-tagged, recombinant extracellular domain, 1 mg (500 μg x 2), HEK293-expressed, low endotoxin, MALS-verified
Recombinant extracellular domain of the human glucagon-like peptide-1 receptor engineered with a C-terminal polyhistidine tag. Expressed in HEK293 cells and verified by SEC-MALS, this lyophilized protein is prepared for low-endotoxin applications and biophysical characterization.
- Expressed in HEK293 cells
- Amino acid range: Ala21-Glu139
- Calculated molecular weight: 15.6 kDa; migrates 25-33 kDa when glycosylated
- Purity greater than 90% by SDS-PAGE and SEC-MALS
- Endotoxin less than 0.01 EU/μg (LAL/rFC)
- Formulated lyophilized from 0.22 μm filtered PBS, pH 7.4, with trehalose
- Available sizes include 100 μg and 1 mg (two 500 μg vials)
- Store lyophilized at -20°C or lower; avoid repeated freeze-thaw cycles
Invent Biotechnologies Inc Minute™ High Fidelity ER Isolation Kit for Liver Tissue - spin column-based (20 Preps)
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for decades. Traditional methods for isolating ER are based on density-gradient ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and time-consuming and contain significant cross-contamination. Currently, all commercial kits for ER isolation are based on methods developed in the 1970s. Unlike any other ER isolation kit on the market, this kit employs a patented spin-column-based technology that is simple and rapid and requires only a small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from frozen liver tissues without using a Dounce homogenizer or ultracentrifugation.
Genscript Corporation CD4/LEU3 His Rhesus macaque
CD4 also known as L3T4 T4 and W3/25 is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes It is a standard phenotype marker for the identification of T cell populations Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses In T-cells functions primarily as a coreceptor for MHC class II molecule peptide complex
Genscript Corporation 4-1BB/TNFRSF9Biotin His A
4-1BB is also known as CD137 is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily CD137 can be expressed by activated T cells but to a larger extent on CD8 than on CD4 T cells In addition CD137 expression is found on dendritic cells B cells follicular dendritic cells natural killer cells granulocytes and cells of blood vessel walls at sites of inflammation
Cellomics Technology LLC LENTIVIRUS TITRATION STRIP (20
Lentivirus titration strip is a quick immunoassay to check lentivirus titers. Imagine how much time and money you could save when you know the lentivirus titers too low to be concentrated before you purify and concentrate the virus.